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KMID : 0380420180420010001
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Journal of Prventive Veterinary Medicine
2018 Volume.42 No. 1 p.1 ~ p.9
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Effects of cryopreservation on equine adipose tissue-derived mesenchymal stem cell plasticity
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Jeong Da-Un
Byeon Jeong-Su
Lee Jienny
Park Mi-Jeong
Gu Na-Yeon
Cho In-Soo
Cha Sang-Ho
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Abstract
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Mesenchymal stem cells (MSCs), which are present in all tissues, can differentiate into cells with various specific functions. Recently, cell-based therapies using MSCs have been increasing in the veterinary research and related fields. In this study, we investigated the cellular morphology, proliferating capacities, expression of cell surface markers such as CD13, CD34, CD44, CD45, CD90, and CD105, mesodermal differentiation potentials, and expression of senescence-related markers of p53, p21, and telomerase reverse transcriptase in equine adipose tissue-derived MSCs (eAD-MSCs) after cryopreservation. The eAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 1 year (< 1 year, G1) and more than 3 years (> 3 years, G2), respectively. After cryopreservation for 1 - 3 years, G2 eAD-MSCs showed similar cellular morphology, proliferating capacities, and expression of cell surface markers when compared with G1 eAD-MSCs. Moreover, cryopreservation did not affect the adipogenic, chondrogenic, or osteogenic differentiation potentials of G1 and G2 eAD-MSCs. Collectively, cryopreservation for (or over) 3 years maintained the stem cell phenotype and differentiation potentials of eAD-MSCs. These results will be an advantage that can be effectively used for future development of cell-based therapies.
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KEYWORD
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Equine adipose tissue, mesenchymal stem cells, cryopreservation, differentiation potentials, cellular senescence
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